A General Synthetic Route to Defined, Biologically Active Multivalent Arrays
نویسندگان
چکیده
A new, general strategy for the synthesis of biologically active multivalent arrays displaying diverse functionality has been developed. This method exploits the ability of ring-opening metathesis polymerizations to produce polymers of defined lengths. By incorporating N-hydroxysuccinimide esters into the polymers, recognition epitopes bearing nucleophilic functional groups, in this case an R-mannose derivative, can be attached. The synthesis, characterization, and biological evaluation of mannose-bearing polymers demonstrated the utility of this new methodology. This strategy will facilitate the creation of diverse multivalent libraries and the large-scale production of multidentate ligands.
منابع مشابه
Synthesis of end-labeled multivalent ligands for exploring cell-surface-receptor-ligand interactions.
BACKGROUND Ring-opening metathesis polymerization (ROMP) is a powerful synthetic method for generating unique materials. The functional group tolerance of ruthenium ROMP initiators allows the synthesis of a wide range of biologically active polymers. We generated multivalent ligands that inhibit cell surface L-selectin, a protein that mediates lymphocyte homing and leukocyte recruitment in infl...
متن کاملInfluence of Natural Sources of Biologically Active Substances on Livestock and Poultry Reproduction
Synthetic hormones and bioactive substances (vitamins, pigments and isoflavons) which have been used in the feed industry are accumulated in milk, meat and eggs. It is suspected that these are responsible for a number of side-effects such as carcinogenesis, liver and kidney damage, allergies in consumers. Furthermore the synthetic hormones, vitamins, pigments are less active than those extracte...
متن کاملOver Expression of Biologically Active Interferon Beta Using Synthetic Gene in E. coli
In this study, our previously reported novel synthetic gene encoding 166 residues of interferon-? was used for an efficient expression of IFN-?. The synthetic gene was cloned into pET21a expression vector and transferred into E. coli. Recombinant protein was over-expressed in the E. coli. Identity of the recombinant protein was confirmed by western blot analysis. The recombinant protein was bio...
متن کاملOn meromorphically multivalent functions defined by multiplier transformation
The purpose of this paper is to derive various useful subordination properties and characteristics for certain subclass of multivalent meromorphic functions, which are defined here by the multiplier transformation. Also, we obtained inclusion relationship for this subclass.
متن کاملOne pot, Five-component Synthesis of Functionalized Piperidines Using Zn(OAc)2.2H2O as a Highly Efficient Catalyst
A convenient synthetic route for the synthesis of biologically active functionalized piperidines usingZn(OAc)2.2H2O as a highly efficient catalyst via five-component reaction of aromatic aldehydes,anilines and β-ketoesters at ambient temperature has studied. Mild reaction conditions, simplicity ofoperation and work-up procedures with no necessity of chromatographic purification steps, theavaila...
متن کامل